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To measure proliferation in postnatal development, we injected an age series of CD1 wild-type mice with a single dose of the thymidine analogue Brd U, 100 min before perfusion (Fig. Immunostaining revealed Brd U cells was greater in basis pontis than in any other region of the brainstem (Fig. 3f,g), and also greater in the pontine tegmentum relative to midbrain tegmentum (Supplementary Fig. We observed three main anatomical compartments of Brd U cells were denser in the basis pontis than in the tegmentum (Fig. This greater proliferation in basis pontis coincides with its larger daily per cent increase in volume (Fig. Basis pontis proliferation peaked at 276±15 cells per mm at P4, and declined more than 50% by P12 (Fig. Similarly, proliferation in VZ and midline peaked at P4 with 40.6±3.1 and 16.3±3.7 cells per mm, respectively, and declined more than 50% by P8 and P12, respectively (Fig. There was a significant increase in proliferation density from P0 to P4 in VZ (P=0.0302, unpaired t-test) and basis pontis (P=0.0072), while in tegmentum and midline the increased density of Brd U VZ cells at P28 or above.These findings show that the pons is the most proliferative postnatal brainstem region; its cell proliferation shows a single postnatal peak, at P4, coincident with the period of fastest growth.We identify three postnatal proliferative compartments: ventricular, midline and parenchymal.We find no evidence of postnatal neurogenesis in the pons, but each progenitor compartment produces new astroglia and oligodendroglia; the latter expand 10- to 18-fold postnatally, and are derived mostly from the parenchyma. The nature of postnatal pontine progenitor cells has consequences for pontine gliomagenesis, and for the normal postnatal development and function of this crucial brain region.Contact us if you experience any difficulty logging in.Bromodeoxyuridine, variously abbreviated as Brd U, Bud R, and Brd Urd, is a halogenated thymidine analog that is permanently integrated into the DNA of dividing cells during DNA synthesis in S phase.
Unpaired t-test revealed significant differences between the following consecutive timepoints: P0 and P4 (both regions), P4 and P10 (both regions), and P10 and P16 (tegmentum). To determine whether myelination accounted for the rapid early growth of the postnatal mouse basis pontis, we performed immunohistochemistry for myelin basic protein (MBP).
The distribution of MBP in pontine sections of increasing age is shown in Fig. MBP appeared in concert with adenomatous polyposis coli (APC), a marker of mature oligodendrocytes, first in selected white matter tracts of tegmentum, for example, medial longitudinal fasciculus and trigeminal nerve (V) by P2–P4, followed by white matter of basis pontis from P8 to P10 and grey matter of basis pontis and tegmentum by P8 to P12 (Fig. The extent of myelination and the apparent thickness of myelinated tracts increased throughout the pons between P10 and P24 (Fig. The progression of myelination evidenced by MBP staining is consistent with a magnetic resonance imaging study on the development of 12 selected white matter tracts in postnatal mouse brain (including three brainstem tracts).